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u937 egfp cell line  (ATCC)


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    ATCC u937 egfp cell line
    Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis <t>by</t> <t>U937-EGFP</t> macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.
    U937 Egfp Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u937 egfp cell line/product/ATCC
    Average 99 stars, based on 6779 article reviews
    u937 egfp cell line - by Bioz Stars, 2026-06
    99/100 stars

    Images

    1) Product Images from "A fully human IgG1 antibody targeting MICA α1 domain inhibits interaction with NKG2D and activates immune effector functions against MICA-expressing cells"

    Article Title: A fully human IgG1 antibody targeting MICA α1 domain inhibits interaction with NKG2D and activates immune effector functions against MICA-expressing cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1740184

    Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis by U937-EGFP macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.
    Figure Legend Snippet: Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis by U937-EGFP macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.

    Techniques Used: Labeling, Cell Culture, Control, Confocal Microscopy



    Similar Products

    u937 egfp cell line  (ATCC)
    99
    ATCC u937 egfp cell line
    Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis <t>by</t> <t>U937-EGFP</t> macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.
    U937 Egfp Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u937 egfp cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    u937 egfp cell line - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis by U937-EGFP macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.

    Journal: Frontiers in Immunology

    Article Title: A fully human IgG1 antibody targeting MICA α1 domain inhibits interaction with NKG2D and activates immune effector functions against MICA-expressing cells

    doi: 10.3389/fimmu.2026.1740184

    Figure Lengend Snippet: Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis by U937-EGFP macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.

    Article Snippet: U937-EGFP cell line, derived from human promonocytic myeloid leukemia (ATCC ® CRL-1593.2, USA), was treated with 10 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, USA) for 48 h to induce macrophage differentiation, following established protocols ( ).

    Techniques: Labeling, Cell Culture, Control, Confocal Microscopy